Development of a Monoclonal Antibody to Reverse Overdose from Fentanyl and Its Analogs: From Manufacturing to Clinical Trials

The widespread availability of fentanyl and other potent synthetic opioids has dramatically increased opioid-related fatal overdoses. This project will develop and manufacture immune molecules (monoclonal antibodies) to reverse and treat overdose from fentanyl by keeping it out of the brain. This research will advance promising results in animal studies (preventing and reversing fentanyl- and carfentanil-induced […]

Parent and teacher engagement as an undervalued implementation determinant

The proposed research addresses three important objectives (1) complete a scoping review to map determinants of teacher implementation of evidence-based practices for child behavior in preschool , (2) identify strategies to improve teacher implementation through a series of casual pathway diagrams, and (3) conceptualize and operationalize strategies with stakeholders to increase feasibility.

Improving diagnostic imaging to guide treatment of neuroinflammation

Infection by West Nile Virus can lead to encephalitis, or harmful inflammation of the brain. The immune system is critical for controlling viral replication and spread early in West Nile Virus infection, but persistent immune activation causes encephalitis that can result in brain damage even after the virus has been cleared. Recent pharmacologic advances have […]

Modeling patient mutations in iPSC-derived neurons to reveal cellular mechanisms of schizophrenia

Schizophrenia is a common and devastating neurodevelopmental disorder characterized by genetic heterogeneity. In this project, genes that are disrupted by rare, damaging mutations in individuals with schizophrenia will be evaluated using CRISPRi in iPSCs. Cell lines will be evaluated for phenotypes relevant to schizophrenia. A patient-derived iPSC line will be created that harbors a mutation […]

Latinx Health in Rural Areas (Project LAHRA)

This project aims to examine health disparities in substance use and factors influencing the health and well-being of Latinx youth residing in rural and small-town communities of the United States, a growing but understudied and underserved population.

Cannabis legalization and changes in young adult substance use, related health risk behaviors, and risk factors in WA State (Project YAM)

This project involves analyses of data from the Washington State Young Adult Health Study to examine the impact of cannabis legalization on cannabis-related risk factors, substance use, and related health risk behaviors such as driving while intoxicated, and to study developmental trajectories of substance use and the role of community level and other cannabis-specific risk […]

Project Reflect

This project examines marijuana-specific self-regulation processes in relation to marijuana use and consequences both across days and throughout the day to identify how psychological states and contextual factors influence these self-regulation processes using an EMA design with surveys four times a day for two weeks.

Microglia and opioid withdrawal: mechanisms of negative reinforcement

Extensive opioid use and repeated abstinence increases the likelihood of severe withdrawal and contributes to the risk of relapse. Previously we found that microglia, the innate immune cells in the brain, have dramatic changes in gene expression and signaling during opioid tolerance and withdrawal. We propose to use fentanyl and a recently developed transgenic mice to […]

Determining if activity in LHb projections to the RMTg promotes cued reinstatement to fentanyl seeking through indirect inhibition of VTA dopamine neurons

The main goals of this proposal are twofold: 1) to develop and optimize a general behavioral and technical platform for studying the functional neurocircuitry underlying opioid use disorder, and 2) to determine if activity in LHb projections to the RMTg promotes cued reinstatement to fentanyl seeking through indirect inhibition of VTA dopamine neurons.

Determining if activity in specific lateral habenula output pathways motivates avoidance of synthetic opioid withdrawal or cue induced reinstatement

Opioid abuse has reached epidemic proportions in the United States and is responsible for more than 40,000 overdose deaths each year. Avoidance of withdrawal and drug-associated cues are key deterrents to voluntary abstinence in humans. By studying the neural circuits responsible for motivating avoidance of physical and emotional pain, as well as those that process […]