My laboratory studies the therapeutic value and molecular mechanism of action of drugs targeting the cannabinoid signaling system. Our goal is to develop novel treatments for brain deficits and cognitive impairment linked to neurodegeneration (Huntington’s disease) and drug addiction. We are particularly interested in the cannabinoid signaling system expressed by medium spiny neurons (MSN), a neuronal cell population affected in both diseases.
To understand how cannabinoid receptors and their endogenous ligands, the endocannabinoids, control fundamental functions of MSN under healthy and disease states, we leverages a broad array of analytical approaches and molecular technics. Thus, over the years, my laboratory has acquired expertise in analytical chemistry (especially measuring drugs and lipids by GC-MS and LC-MS, and more recently proteins by SILAC), pharmacology (especially the pharmacodynamics characteristics of compounds acting at GPCR), biochemistry (second messengers and enzymatic activities), molecular biology (subcloning and genetic manipulations of protein expression), cell phenotype (gene arrays and qPCR), cell viability (both using metabolism and cell integrity-based assays), semi-quantitative immunocytochemistry and immunohistochemistry, pharmacokinetics, safety and toxicology and animal behavior (motor and motivated: e.g. drug sensitization and CPP).
Front Mol Neurosci 12(): 286
van Esbroeck ACM, Kantae V, Di X, van der Wel T, den Dulk H, Stevens AF, Singh S, Bakker AT, Florea BI, Stella N, Overkleeft HS, Hankemeier T, van der Stelt M
(2020 Feb 13)
J Med Chem 63(3): 1261-1280
Castelli R, Scalvini L, Vacondio F, Lodola A, Anselmi M, Vezzosi S, Carmi C, Bassi M, Ferlenghi F, Rivara S, Møller IR, Rand KD, Daglian J, Wei D, Dotsey EY, Ahmed F, Jung KM, Stella N, Singh S, Mor M, Piomelli D
Neurobiol Dis 132(): 104607
Cao JK, Viray K, Zweifel L, Stella N
Glia 67(8): 1558-1570
Cherry AE, Vicente JJ, Xu C, Morrison RS, Ong SE, Wordeman L, Stella N
Neuropsychopharmacology 44(8): 1406-1414
Kruse LC, Cao JK, Viray K, Stella N, Clark JJ
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